核转录因子SOX4抗体-认证认可-资讯-生物在线

核转录因子SOX4抗体

作者:上海雅吉生物科技有限公司 2020-11-09T00:00 (访问量:1391)

 

中文名称 核转录因子SOX4抗体
别    名 SOX4; SOX 4; SOX-4; Ecotropic viral integration site 16; EVI 16; EVI16; SRY (sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; SOX4_HUMAN.  
研究领域 神经生物学  干细胞  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Chicken, )
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 52kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SOX4:54-103/474 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Function:
The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. SOX4 shows a high degree of sequence homology with SOX11. Both are expressed in the CNS. SOX4 and SOX11 expression increase together in the developing CNS (with some exceptions: external granule layer of the cerebellum). SOX4 knock-out mice have been shown to die of a heart defect half way through gestation with no observable CNS phenotype, although it has been proposed that SOX11 compensates for the absence of SOX4.

Subunit:
Interacts with UBE2I.

Subcellular Location:
Nuclear

Tissue Specificity:
Testis, brain, and heart.

Similarity:
Contains 1 HMG box DNA-binding domain.

SWISS:
Q06945

Gene ID:
6659

Database links:

Entrez Gene: 395143 Chicken

Entrez Gene: 768313 Cow

Entrez Gene: 6659 Human

Entrez Gene: 20677 Mouse

Entrez Gene: 364712 Rat

Entrez Gene: 336346 Zebrafish

Omim: 184430 Human

SwissProt: Q0VCF8 Cow

SwissProt: Q06945 Human

SwissProt: Q06831 Mouse

Unigene: 643910 Human

Unigene: 240627 Mouse

Unigene: 455819 Mouse

Unigene: 163667 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Sample:
Testis (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SOX4 (bs-11208R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIAE) Polyclonal Antibody, Unconjugated (bs-17480R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-SOX4 antibody (bs-11208R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-APC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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